Identifying crossover locations in Arabidopsis thaliana recq4a recq4b and HEI10 recq4a recq4b Col x Ler F2 populations using genotyping by sequencing. Identifying crossover locations in Arabidopsis thaliana recq4a recq4b and HEI10 recq4a recq4b Col x Ler F2 populations using genotyping by sequencing

Genomic DNA from 192 recq4a recq4b and 192 HEI10 recq4a recq4b Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Ziolkowski et al, 2017 Genes & Dev). Sequencing data was analysed to identify crossovers as previously reported, using the TIGER pipeline (Rowan et al, 2015 G3 (Bethesda); Yelina et al, 2015 Genes & Dev).