Microbial assemblages and functional predictions in organic-rich subterranean estuaries

Microbial communities in subterranean estuaries (STE) mediate biogeochemical reactions of coastal groundwater discharging into the oceans; however, studies on their response to abrupt environmental changes caused by climate and land use alterations are still limited. In this study, we conducted a controlled laboratory study using combined geochemical and metagenomic approaches to investigate microbial structures and their metabolic pathways under different nitrate (NO3-) inputs, saline solutions, and incubation times. This is a supplementary data to manuscript "Microbial assemblages and functional predictions in organic-rich subterranean estuaries: impact of climate and land use changes" submitted to Journal of Geophysical Research: Biogeosciences.