Reaction conditions were adapted to CrPV IGR IRES-mediated translation in eukaryotic cell-free systems.
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Reaction parameters used for cap-dependent translation in coupled reactions (left) were optimized for CrPV IGR IRES-mediated translation (right). Optimization was performed by cell-free expression of active LUC using the pIX3.0-CrPV IGR IRES (GCT) vector as a template. Reactions were incubated for 3 h in a thermomixer.
创建时间：
2015-12-02
