Metagenomics sequences of hot water samples from simulated hot water premise plumbing

A simulated hot water premise plumbing system was constructed to examine the impact of several factors (e.g., water heater temperature setting, stagnation, and the length of stagnation) on hot water microbiome and specifically opportunistic pathogens. A total of 12 water samples were selected for shotgun metagenomic sequencing using Illumina HiSeq 2500. These samples included a influent cold water, recirculating hot waters, and stagnated hot water in distal taps from an experimental rig run with increasing temperature settings from 39°C for 5 months to 42°C and then 51°C for two months each, and from a control rig run constantly at 39°C.