Quantification of <i>yurJ</i> and <i>msmX</i> mRNA level in the <i>B</i>. <i>subtilis</i> wild-type strain.

The results represent the fold-change of the expression in the target conditions (T) versus the control conditions (C). (glu), (ara) and (A3) indicate the presence of 0.1% of glucose, arabinose or arabinotriose, respectively. Primers ARA638 and ARA639 were used for yurJ and primers ARA640 and ARA641 for msmX. Fold-change was normalized using primers ARA583 and ARA584 for the 16S gene. Statistical analyses were performed with GraphPad Prism (version 5.00) using Ct values obtained from three independent assays. p values were determined using an unpaired t test (ns, non-significant difference; *, p < 0.05; **, p < 0.01; ***, p < 0.001).