The role of RUNX2 and BHLHE40 in pathologically relevant CD4-positive tissue-resident T-cells in Crohn's disease. (CITE-seq, CD4+ T cell) II

Tissue-resident T-cells (TRM) are deeply involved in immune memory at the site of inflammation. Here, we identified two key transcription factors, RUNX2 and BHLHE40 as regulators of pathologically relevant CD4-positive TRM in the inflamed gut mucosa of Crohn’s disease patients. CD4+ T cells were sorted from mononuclear cells derived from surgically resected fresh gut specimens, peripheral, blood, mesenteric lymph nodes by flow cytometry. Each specimen was labeled with HTO antibodies and subjected to single-cell CITE-seq analysis.