Live-cell imaging data set of HaCaT FUCCI cells with BF/DIC and fluorescence

This dataset contains time-lapse acquisitions of HaCaT FUCCI cells cultured in 8-well chambered coverslips with fluorobrite media. Cells were provided by Dr. Francesco Pasqualini and Dr. Moises di Sante (http://www.syntheticphysiologylab.com), and videos acquired at the Henriques Lab (https://henriqueslab.org/). HaCaT_FUCCI_BF-live set: Nikon Ti2, Channels BF (16 bit), FITC (12 bit sensitive) and Cy5 (12 bit sensitive). 20 positions, acquired in Nikon Ti2 with a 20x/0.8, every 30 minutes for 18 hours. HaCaT_FUCCI_DIC-live set: Nikon Ti2, Channels DIC, FITC and Cy5 (all 12 bit sensitive). 20 positions, acquired in Nikon Ti2 with a 20x/0.8, every 30 minutes for 18 hours.