Global changes in granulosa cell transcription in response to the LH surge

During ovulation, the LH surge leads to the activation of various signalling cascades in granulosa cells, resulting in a critical shift in chromatin landscape and correspondingly the ovarian gene expression profile. Such large-scale genomic reprogramming involves a specific suite of ovulatory transcription factors. This study aims to characterise global changes in the chromatin landscape that are induced by the LH surge in granulosa cells during ovulation. Overall design: RNA-seq was performed on 4 replicates of granulosa cells obtained from eCG-stimulated mice after 0 h or 8 h post-hCG treatment