ChIP-Seq Transcription Factor Data

We developed a method, ChIP-sequencing (ChIP-seq), combining chromatin immunoprecipitation (ChIP) and massively parallel sequencing to identify mammalian DNA sequences bound by transcription factors in vivo. We used ChIP-seq to map STAT1 targets in interferon-gamma (IFN-gamma)-stimulated and unstimulated human HeLa S3 cells, and compared the method's performance to ChIP-PCR and to ChIP-chip for four chromosomes.For both Chromatin- immunoprecipation Transcription Factors and Histone modifications. Sequence files and the associated probability files are also provided.

本研究团队研发了一种结合染色质免疫沉淀（ChIP）与大规模并行测序的ChIP-sequencing（ChIP-seq）方法，旨在鉴定哺乳动物细胞中由转录因子在体内结合的DNA序列。本研究利用ChIP-seq技术对受干扰素-γ（IFN-gamma）刺激及未刺激的人HeLa S3细胞中的STAT1靶点进行定位，并将该方法在四条染色体上的表现与ChIP-PCR及ChIP-chip进行比较，涉及染色质免疫沉淀转录因子与组蛋白修饰。此外，我们还提供了序列文件及其相关概率文件。