Sterols affect oil droplet assemble

We carried out a forward genetic screen using a transgenic line constitutively overproducing a GFP-tagged OLEOSIN1 fusion protein and searched for mutants with the altered size, number, and morphology of leaf LDs. We identified and characterized one of the mutants, M1-7, that is severely defective in the accumulation of LDs in leaves. M1-7 mutant was outcrossed to ecotype Landsberg to generate a mapping population that was used for rough mapping, and the mutant locus was delimited to an ~ 1500-kb region in the chromosome 1. This project is arm to identify the mutation that cause the phenotype of M1-7 through whole genomic DNA sequencing using DNA from plants have phenotypes and those do not have phenotypes.