Data from: Phage-displayed synthetic library and screening platform for nanobody discovery-source data

Nanobodies, single-domain antibodies derived from camelid heavy-chain
antibodies, are known for their high affinity, stability, and small size,
which make them useful in biological research and therapeutic
applications. However, traditional nanobody generation methods rely on
camelid immunization, which can be costly and time-consuming, restricting
their practical feasibility. In this study, we present a phage-displayed
synthetic library for nanobody discovery. To validate this approach, we
screened nanobodies targeting various Drosophila secreted proteins. The
nanobodies identified were suitable for applications such as
immunostaining and immunoblotting, supporting the phage-displayed
synthetic library as a versatile platform for nanobody development. To
address the challenge of limited accessibility to high-quality synthetic
libraries, this library is openly available for non-profit use.