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Figure 7- Masson trichrome staining and histomorphometric analysis of defects treated with DBBM and SSB. (a) Representative Masson trichrome staining images of defects with different treatments at postoperative 2 weeks and 4 weeks. White dash line indicates the border of the defect. While triangle indicates the material remnants; White arrow indicates the newly formed bone. Scale bar was 1 mm and 200 μm for low and high magnification, respectively; (b-c) Histomorphometric analysis for the amount of new bone formation using Masson trichrome staining images at postoperative 2 weeks (b) and 4 weeks (c). The amount of bone formation was determined = The bone formation area in the defect area (μm<sup>2</sup>) / cross-sectional distance (μm). Student’s t test was used for the statistical analysis. ns indicates no significance. **p < 0.01.