Distribution of total histone variants H3.1 and H3.3 in pluripotent mouse embryonic stem cells (mESCs) and in vitro differentiated neuronal precursor cells (NPCs)

We profiled the enrichment of the replicative H3.1 and non-replicative H3.3 histone variants using the SNAP-seq assay previously developed in the team (Gatto et al., 2022, Forest et al., 2023). We used KH2 mouse embryonic stem cells (mESCs) bearing doxycycline-inducible H3.1/H3.3-SNAP, which were expressed at low levels following 48h doxycycline treatment. To understand how the variants are distributed cell types of different potencies, we assayed them in pluripotent mESCs and following a 7-day differentiation into neural precursor cells (NPCs).