Single-nucleus RNA-seq of NASH HCC liver tissue

Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer-related death world-wide. The prevalence of non-alcoholic fatty liver disease (NAFLD) has been rising, along with an increase in NAFLD-related HCC. To investigate cell-type composition in NAFLD and HCC, we performed single-nucleus RNA-seq (snRNA-seq) of patients with fatty liver disease in order to transcriptionally characterize cell-types in an unbiased manner. Out data reveal a large amount of heterogeneity in hepatocyte populations, as well as recovering all expected liver cell-types. Snap-frozen archived liver biopsies from tumor and adjacent non-tumor resections were processed from 3 patients with fatty liver disease and HCC (n=3 paired; n=6 total). Nuclei were isolated into a suspension and immediately processed with the 10X Chromium platform following the Single Cell 3' v3 protocol for library preparation. Libraries were then sequenced on an Illumina novaSeq 6000. ***Please note that raw data is not provided due to human subject privacy concerns***