human gut metagenome Genome sequencing. human gut metagenome

The objective of this study was to analyze the gut microbiota composition on breastfed preterm infants under 1500 grams using 16S rRNA gene sequencing. To perform the intestinal microbiota analysis, total DNA was obtained from each stool sample. A polymerase chain reaction (PCR) was carried out to produce millions of amplicons of the 16S rRNA gene. These amplicons were subjected to massive amplification to generate the nucleotide sequences necessary for microbial analysis. The 16S rRNA gene sequences were processed using advanced bioinformatics to gain insight into the effect of the breast milk intervention on the fecal microbiota in newborns. A predictive approach was used to determine the functional profile of fecal microbial communities, adding valuable information to the role of the gut microbiota in gut health.