m6A methyltransferase Mettl3 is essential for neuronal homeostasis and sleep via modulating Npy in mice. m6A methyltransferase Mettl3 is essential for neuronal homeostasis and sleep via modulating Npy in mice

To unravel the underlying mechanisms how neuronal Mettl3 loss induces the activation of microglia, we performed RNA-sequencing (RNA-seq) with the thalamic tissues of 2- and 4-month-old Ctrl and cKO mice, respectively. Overall design: we performed bulk RNA sequencing (RNA-seq) with both ctrl and cKO mice brain in 2month and 4month to reveals the altered gene expression relating to microglia