Glucose stimulation and oligomycin inhibition of INS-1 832/13 cells followed using the absolute ΔψP and ΔψM assay.
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(A) ΔψP and (B) ΔψM were assayed by measuring whole-well fluorescence of TMRM and PMPI in a microplate reader. Cultures were stimulated with glucose (10 mM) and treated with oligomycin (2 μg/ml) as indicated. Fluorescence traces were calibrated based on the same principles as shown in Fig 2; the time courses are shown truncated before calibration. Data are mean±SE of n = 10 independent experiments.
创建时间：
2016-07-21
