Microarray gene expression profiling of rat cerebellar Granule Cells (GCs) before and after Lamin A-knockdown

Nuclear lamins are nuclear type V intermediate filament proteins that form a meshwork structure underlying the inner nuclear membrane, the nuclear lamina. The nuclear lamina associates with other nuclear envelope proteins and plays numerous roles, including maintaining the nuclear shape and structure, assembly and disassembly of the nucleus, heterochromatin organisation, transcriptional regulation and other nuclear functions. Here, we studied the role of A-type lamins in the maturation of rat cerebellar GCs during normal brain development by performing the gene expression profiling of GCs knocked-down for Lamin A. We demonstrated that Lamin A/C has a key role in neural differentiation and GC maturation, also under physiological conditions. By using Agilent Whole Rat Genome 4x44K microarray platform (grid ID 014879), we performed the gene expression profiling of Lmna-knockdown GCs (n=2) as compared to mock GCs (n=2).