Huntingtin-RNA complex generation for cryoEM analysis 2019/07/24
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https://zenodo.org/record/3382303
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Project: High resolution structural analysis of HTT-nucleic acid complexes
Experiment: Huntingtin-RNA complex generation for cryoEM analysis
Date: 2019/07/24
Background: Huntingtin has been shown to copurify with nucleic acid material as well as bind different nucleic acid species in band shift assays. To more thoroughly characterise this interaction, HTT-nucleic acid complex generation and structure solution should provide insight into how huntingtin performs this function.
Rationale: Previous attempts to capture HTT-nucleic complexes have been difficult and maintaining the complex throughout purification of the sample has been tricky. These four approaches are different ways to try and form a stable complex between Q54 HTT-HAP40 and CAG8RNA.
创建时间：
2024-07-22
