Huntingtin-RNA complex generation for cryoEM analysis 2019/07/24

Project: High resolution structural analysis of HTT-nucleic acid complexes Experiment: Huntingtin-RNA complex generation for cryoEM analysis Date: 2019/07/24 Background: Huntingtin has been shown to copurify with nucleic acid material as well as bind different nucleic acid species in band shift assays. To more thoroughly characterise this interaction, HTT-nucleic acid complex generation and structure solution should provide insight into how huntingtin performs this function. Rationale: Previous attempts to capture HTT-nucleic complexes have been difficult and maintaining the complex throughout purification of the sample has been tricky. These four approaches are different ways to try and form a stable complex between Q54 HTT-HAP40 and CAG8RNA.