Behavioral discrimination and olfactory bulb encoding of odor plume intermittency

Mice were tested to see if they could discriminate different levels of odor intermittency (an odor plume property). Labview was used to run the tests. The RedShirtImaging Neuroplex and NeuroCCD system was used for concomitant optical imaging of the dorsal olfactory bulb in the head-fixed mice. Half the mice expressed G-CamP6f in olfactory receptor neurons, the other half in post-synaptic mitral/tufted cells. Matlab was used to analyze the behavioral and neural glomerular data. Another set of mice was similarly optically imaged under anesthesia to systematically assess neural coding across the dorsal olfactory bulbs of odor intermittency as a function of sniff rate.