Transcriptional dysregulation impairs efficacy of neural stem cell activation during aging [scRNA-seq]

To gain insight of the decreasing activation efficacy in the aging NSCs, we performed single-cell transcriptomics analysis using the Chromium Single Cell 3' platform (10x Genomics). Primary NSCs were isolated from the subventricular zone of the lateral ventricles (SVZ) in the brains of the young and aged mice, respectively. The isolated cells were used in scRNAseq analysis.为探究衰老神经干细胞（Neural Stem Cells, NSCs）激活效能下降的内在机制，我们采用10x Genomics公司的Chromium单细胞3'端转录组平台开展单细胞转录组学分析。我们分别从年轻小鼠与衰老小鼠的侧脑室室管膜下区（SVZ）分离得到原代神经干细胞，并将所分离的细胞用于单细胞RNA测序（single-cell RNA sequencing, scRNAseq）分析。