LAI-1-dependent redistribution of IQGAP1 does not require Cdc42.

(A) A549 cells were treated with siRNA against Cdc42 for 2 days, then with LAI-1 (10 μM, 1.5 h) and protein production or the subcellular localization of the IQGAP1 scaffold protein (green) and the small GTPase Cdc42 (red) was analyzed by confocal microscopy using antibodies against IQGAP1 or Cdc42. Nuclei were stained with DAPI (blue). (B) Quantification of protein production in A549 cells after RNAi treatment (percent cells producing protein of interest; n = 50). Means and standard deviations of 3 independent experiments are shown (***p < 0.001). (C) Redistribution of IQGAP1 in absence of Cdc42 upon LAI-1 treatment. The graph is based on the relative fluorescence intensity along cell sections (n = 50). Means and standard deviations of 3 independent experiments are shown (***p < 0.001). Bar, 20 μm.