Bacillus cereus Raw sequence reads

RNAs can begin to fold immediately after emerging from RNA polymerase during transcription. Interactions between nascent RNAs and ligands during cotranscriptional folding can direct the formation of alternative RNA structures, a feature exploited by non-coding RNAs called riboswitches to make gene regulatory decisions. These sequencing datasets were generated using cotranscriptional SHAPE-Seq which measures structural information of nascent RNAs during transcription. The datasets describe the in vitro folding pathways of the B. cereus Fluoride riboswitch using a biotin-streptavidin RNA polymerase roadblocking strategy.