Effect of depletion of OGT on gene expression during M2 polarization of macrophages

To investigate the function OGT in the regulation of M2 polarization of macrophages, we established IL-4-activated bone marrow-derived macrophages (BMDMs) from mice of wild-type control or Lyz2-Cre/Loxp-mediated knockout of OGT. We then performed gene expression profiling analysis using data obtained from RNA-seq of wild-type (WT) and OGT-knockout (OGT-KO) macrophages at two time points during IL-4 (20ng/µl) sitmulation. Overall design: Comparative gene expression profiling analysis of RNA-seq data for WT and OGT-KO BMDMs.