RNA-sequencing of RNAs found on eRpL22 paralogue-specific ribosomes in Drosophila melanogaster testis

This study uncovers a population of eRpL22 paralogue-specific specialized ribosomes in the fly testis. Wildtype testes were used to isolate eRpL22 paralogue-specific ribosomes complexed with RNA molecules. RNA-sequencing determined the distribution of RNAs (mRNA and ncRNA) on each type of eRpL22 paralogue-specific ribosome, revealing unique translatomes for both eRpL22- and eRpL22-like- ribosomes, as well as an overlapping translatome. Overall design: Examination of RNAs associated with two different ribosome population denoted by eRpL22 paralogues in Drosophila melanogaster testis