Next Generation Sequencing Facilitates Quantitative Analysis of primary myoblast cell treated with different concentration glycine Transcriptomes

Purpose: The goals of this study are to analyze primary myoblast cell from mdx mice skeletal muscle transcriptome profiling (RNA-seq) treated with different concentration glycine. Methods: primary myoblast cell isolated from 6-8-week-old mdx mice limb skeletal muscle and the primary myoblast treated with 0.2mM or 0.8mM glycine 24-hour. Primary myoblast mRNA profiles of 0.2mM glycine treated(3 replicates) and 0.8mM glycine treated(6 replicates) were generated by deep sequencing, using Illumina HiSeq 2500. The sequence reads that passed quality filters were analyzed at the gene level using HISAT2 and StringTie followed by DESeq2. Primary myoblast mRNA profiles of 0.2mM glycine treated(3 replicates) and 0.8mM glycine treated(6 replicates) were generated by deep sequencing, using Illumina HiSeq 2500