<i>H. seropedicae</i> EPS is required for biofilm formation on glass fiber.

H. seropedicae strains were grown in the presence of glass fiber and purified wild type EPS (100 µg.mL−1) when indicated. After 12 hours, bacteria attached to the fiber were stained with crystal violet, washed and de-stained with absolute ethanol. The absorbance of the ethanol (550 nm) was determined and subtracted from the absorbance of the control without bacteria. Different letters indicate significant difference (p<0.001, Duncan multiple range test) between biofilm formation by the strains. H. seropedicae EPS is required for biofilm formation on glass fiber.