The sequences of the TA-cloned PCR products for the validation of Insertion sequences associated with xenobiotic degradation genes.
收藏科学数据银行2024-11-18 更新2026-04-23 收录
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资源简介：
This dataset contains the sequences of TA-cloned PCR products for the validation of IS.XDG-Chip. This chip is a high-throughput quantitative PCR (HT-qPCR) platform designed to detect and quantify xenobiotic degradation genes (XDGs) in diverse environmental samples. A total of 9 primer sets were validated for their high specificity. The universal primers barcoding bacterial 16S rRNA gene were included as a reference gene of IS.XDG-Chip. Specific primers were designed for each target gene, followed by PCR amplification. Amplicons displaying single bands of the expected fragment size were selected and subjected to T-A cloning. For each target, ten clones were randomly selected for Sanger sequencing.
提供机构：
Institute of urban environment; University of Chinese Academy of Sciences; Institute of Urban Environment
创建时间：
2024-11-08
