Randomized PAM depletion of AsCas12a
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https://www.ncbi.nlm.nih.gov/sra/SRP172754
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资源简介：
We report the PAMs of AsCas12a using a cell-free TXTL-based cleavage assay. By adding randomized PAM library and AsCas12a-gRNA in vitro, functional PAM sequences were cleaved, while non-functional PAMs remained. By amplifying the non-cleaved DNA, we use next-generation sequencing to analyze the depletion of functional PAMs of AsCas12a. Overall design: Uncleaved DNA from the randomized PAM library was amplified using Illumina Nextera Indices
创建时间：
2019-09-24
