Expression data from Arabidopsis thaliana mutants and treatments

Plants need to be able to respond to changes quickly due to the inability of a plant to change location. Hormones within the plant signal for these transcriptional changes that will affect the plant's ability to survive. Strigolactone is a plant hormone that was more recently discovered so has a less detailed understanding of what genes it regulates, compared to other plant hormones. First published in Brewer et al 2016, PNAS We used microarrays to determine transcriptional responses in strigolactone mutants and in wild-type plants with various physiological treatment which affect hormone levels over 24 hours. Two-week old Arabidopsis thaliana plants were either kept intact, decapitated (shoot tip was removed), decapitated and treated with IAA, treated with NPA. Additionally Arabidopsis thaliana plants with mutations in the MAX1, MAX2, or MAX3 gene were profiled. Plant hypocotyls were harvested 24 h after treatment and 120-150 individual hypocotyls were harvested for one biological replicate. Three biological replicates were conducted for each of the seven conditions