Gene expression profile of goblet cells (GCs) from the distal colon (DC) and the 8th portion of the small intestine (Si8)

Goblet cells together other cell types make up the intestinal epithelium and are responsible for mucus production. The goal of this project is to fully characterize the gene expression profile of the GCs in the colon and small intestine. Overall design: Intestinal GCs and the remaining epithelial cells (ECs) of the RedMUC298trTg mice were isolated by Fluorescence-activated cell sorting (FACS) according to the presence or absence of mCherry signal. Sorted GCs were analysed using RNA sequencing (RNAseq).