S1 Data - Suppression of mechanical hypersensitivity and change in the expression of the dopamine D2 receptor by administration of anti-CGRP antibody into the trigeminal ganglion in trigeminal neuropathic pain model rats

S1 Fig. Photomicrograph of phosphorylated extracellular signal-regulated kinase (pERK)-immunoreactive (-IR) neurons in the trigeminal spinal subnucleus caudalis (Vc) of a sham rat. S2 Fig. Photomicrograph of pERK-IR neurons in the Vc of a chronic constriction injury of the infraorbital nerve (ION-CCI) rat. S3 Fig. Photomicrograph of pERK-IR neurons in the Vc of an ION-CCI rat receiving a vehicle. S4 Fig. Photomicrograph of pERK-IR neurons in the Vc of an ION-CCI rat receiving calcitonin gene-related peptide (CGRP). S5 Fig. Photomicrograph of pERK-IR neurons in the Vc of an ION-CCI rat receiving control immunogloublin G (IgG). S6 Fig. Photomicrograph of pERK-IR neurons in the Vc of an ION-CCI rat receiving an anti-CGRP antibody. S7 Fig. High magnification photomicrograph of pERK-IR neurons in the Vc of a sham rat. S8 Fig. High magnification photomicrograph of pERK-IR neurons in the Vc of an ION-CCI rat. S9 Fig. High magnification photomicrograph of pERK-IR neurons in the Vc of an ION-CCI rat receiving a vehicle. S10 Fig. High magnification photomicrograph of pERK-IR neurons in the Vc of an ION-CCI rat receiving CGRP. S11 Fig. High magnification photomicrograph of pERK-IR neurons in the Vc of an ION-CCI rat receiving control IgG. S12 Fig. High magnification photomicrograph of pERK-IR neurons in the Vc of an ION-CCI rat receiving an anti-CGRP antibody. S13 Fig. Immunofluorescent image of pERK in the Vc. S14 Fig. Immunofluorescent image of neuronal nuclei (NeuN) in the Vc. S15 Fig. Merged image of pERK and NeuN in the Vc. S16 Fig. Immunofluorescent image of pERK in the Vc. S17 Fig. Immunofluorescent image of dopamineD2 receptor (D2 receptor) in the Vc. S18 Fig. Merged image of pERK and D2 receptor in the Vc. S19 Fig. Immunofluorescent image of receptor activity modifying protein 1 (RAMP1) in the Vc. S20 Fig. Immunofluorescent image of D2 receptor in the Vc. S21 Fig. Merged image of RAMP1 and D2 receptor in the Vc. S22 Fig. Photomicrograph of CGRP-IR neurons in the trigeminal ganglion (TG) of a sham rat. S23 Fig. Photomicrograph of CGRP-IR neurons in the TG of an ION-CCI rat. S24 Fig. Photomicrograph of CGRP-IR neurons in the TG of an ION-CCI rat receiving vehicle. S25 Fig. Photomicrograph of CGRP-IR neurons in the TG of an ION-CCI rat receiving CGRP. S26 Fig. Photomicrograph of CGRP-IR neurons in the TG of an ION-CCI rat receiving control IgG. S27 Fig. Photomicrograph of CGRP-IR neurons in the TG of an ION-CCI rat receiving an anti-CGRP antibody. S28 Fig. Immunofluorescent image of glial fibrillary acidic protein (GFAP) in the TG of a sham rat. S29 Fig. Immunofluorescent image of GFAP in the TG of an ION-CCI rat. S30 Fig. Immunofluorescent image of GFAP in the TG of an ION-CCI rat receiving a vehicle. S31 Fig. Immunofluorescent image of GFAP in the TG of an ION-CCI rat receiving CGRP. S32 Fig. Immunofluorescent image of GFAP in the TG of an ION-CCI rat receiving control IgG. S33 Fig. Immunofluorescent image of GFAP in the TG of an ION-CCI rat receiving an anti-CGRP antibody. S34 Fig. Immunofluorescent image of CGRP in the Vc of a sham rat. S35 Fig. Immunofluorescent image of CGRP in the Vc of an ION-CCI rat. S36 Fig. Immunofluorescent image of CGRP in the Vc of an ION-CCI rat receiving a vehicle. S37 Fig. Immunofluorescent image of CGRP in the Vc of an ION-CCI rat receiving CGRP. S38 Fig. Immunofluorescent image of CGRP in the Vc of an ION-CCI rat receiving control IgG. S39 Fig. Immunofluorescent image of CGRP in the Vc of an ION-CCI rat receiving an anti-CGRP antibody. S40 Fig. Immunofluorescent image of CGRP in the Vc. S41 Fig. Immunofluorescent image of D2 receptor in the Vc. S42 Fig. Merged image of CGRP and D2 receptor in the Vc. S43 Fig. High magnification immunofluorescent image of CGRP in the Vc. S44 Fig. High magnification immunofluorescent image of D2 receptor in the Vc. S45 Fig. High magnification merged image of CGRP and D2 receptor in the Vc. S46 Fig. Immunofluorescent image of D2 receptor in the Vc of a sham rat. S47 Fig. Immunofluorescent image of D2 receptor in the Vc of an ION-CCI rat. S48 Fig. Immunofluorescent image of D2 receptor in the Vc of an ION-CCI rat receiving a vehicle. S49 Fig. Immunofluorescent image of D2 receptor in the Vc of an ION-CCI rat receiving CGRP. S50 Fig. Immunofluorescent image of D2 receptor in the Vc of an ION-CCI rat receiving control IgG. S51 Fig. Immunofluorescent image of D2 receptor in the Vc of an ION-CCI rat receiving an anti-CGRP antibody. S52 Fig. High magnification immunofluorescent image of D2 receptor in the Vc of a sham rat. S53 Fig. High magnification immunofluorescent image of D2 receptor in the Vc of an ION-CCI rat. S54 Fig. High magnification immunofluorescent image of D2 receptor in the Vc of an ION-CCI rat receiving a vehicle. S55 Fig. High magnification immunofluorescent image of D2 receptor in the Vc of an ION-CCI rat receiving CGRP. S56 Fig. High magnification immunofluorescent image of D2 receptor in the Vc of an ION-CCI rat receiving control IgG. S57 Fig. High magnification immunofluorescent image of D2 receptor in the Vc of an ION-CCI rat receiving an anti-CGRP antibody. S58 Fig. Immunofluorescent image of D2 receptor in the Vc. S59 Fig. Immunofluorescent image of NeuN in the Vc. S60 Fig. Merged image of D2 receptor and NeuN in the Vc. S61 Fig. Immunofluorescent image of D2 receptor in the Vc of an ION-CCI rat. S62 Fig. Immunofluorescent image of D2 receptor in the Vc of an ION-CCI rat receiving a vehicle. S63 Fig. Immunofluorescent image of D2 receptor in the Vc of an ION-CCI rat receiving CGRP. S64 Fig. Immunofluorescent image of D2 receptor in the Vc of an ION-CCI rat receiving control IgG. S65 Fig. Immunofluorescent image of D2 receptor in the Vc of an ION-CCI rat receiving an anti-CGRP antibody. S1 File. Raw data of behavioral and immunohistochemical responses.
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