Genome-wide maps of chromatin state, transcription factor and cofactor occupancy in 5 leiomyoma (MED12 G44 mutant) and 5 matched normal myometrium (WT) patient tissue samples.

We report the application of high resolution MNase ChIP for high-throughput profiling of H3K27Ac and RNAPII in uterine fibroid tumors. Analysis revealed H3K27Ac remodeling at distal enhancer regions, highlighting the enrichment of AP-1 complex DNA binding motifs. ChIP-sequencing of AP-1 subunits JUN and FOS revealed differential occupancy of AP-1 at enhancer sites. In addition, ChIP-seq of CDK8 submodule subunits CDK8 and MED12 demonstrate differential CDK8 submodule occupancy consistent with remodelled enhancer regions. Examination of the genome-wide histone modification H3K27Ac signal as well as RNAPII occupancy in fibroid tumors and matched normal myometrium. Genome-wide occupancy of AP-1 complex transcription factors JUN and FOS was investigated. In addition, CDK8 submodule factors CDK8 and MED12 were also analyzed in fibroid tumors and matched normal myometrium.