Catalysis of Ac-DEVD-AMC by caspase-3 (dataset formatted for analysis by interferENZY)

Main description

This dataset depicts the catalysis of the fluorogenic substrate Ac-DEVD-AMC to the fluorescent substrate AMC by recombinant purified caspase-3, for fixed concentration of enzyme and variation of concentration of initial substrate. It was originally documented in Biophysical Chemistry 252 (2019) 106193 (https://doi.org/10.1016/j.bpc.2019.106193), and then used as a study case for the webserver interferENZY (a web-based tool for enzymatic assay validation and standardized kinetic analysis; visit https://interferenzy.i3s.up.pt for more information). To this end, it was converted to the format here presented: tab-separated *.txt input required for interferENZY analysis.

Dataset organization

Line 1: Tab-separated initial concentrations of substrate Ac-DEVD-AMC in micromolar (µM) concentration

Line 2: Concentration of enzyme (1 U)

Line 3: Units of time

Line 4: Units of concentration for substrate values and measurements

Line 5: Dataset name

Line 6 and downwards: Tab-separated column-pairs of the progress curves (time,Product) corresponding to the indicated values of initial concentrations of substrate in line 1

Contact information:

Maria Filipa Pinto ([e-mail])
Pedro M. Martins ([e-mail])

i3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal. Telephone number: [phone]