Expression of an Hbr1 C-terminal TAP tag in <i>C</i>. <i>albicans</i>.
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Four URA+ isolates of C. albicans strain BWP17 transfected with an HBR1-TAP-URA3 cassette were tested for fusion protein expression using polyclonal antisera CAB1001 (Thermo Scientific). Lysates in lanes 1,2 and 4 were positive for the 48662 Dalton fusion protein that migrates slower than its calculated molecular weight as described previously [20]. (EPS)
创建时间:
2015-06-03



