Single Cell RNA-seq of the draining lymph nodes of mice treated with either 3M052 or Yellow Fever Vaccine (YF)

Purpose: To understand the innate immune response to an adjuvant, 3M052, and yellow fever vaccine, YFV Methods: Draining lymph nodes were negatively selected for CD19+ and CD3+, then flow sorted into four populations: Dendritic cells (DCs), Double positive cells (DP, CD11b+BST1+), Ly6c+ cells (Ly6c), and plasmacytoid dendritic cells (pDCs). Lymph nodes were harvested at baseline (D0), 24 hours post-treatment (D1) or 28 days post-treatment (D28). Results: TBD Conclusions: TBD Overall design: scRNA of murine lymph nodes after treatment with the adjuvant 3M052 or Yellow Fever vaccine.