Table2_Genome-wide comparison and in silico analysis of splicing factor SYF2/NTC31/p29 in eukaryotes: Special focus on vertebrates.xlsx

The gene SYF2—an RNA splicing factor—can interact with Cyclin D-type binding protein 1 (GICP) in many biological processes, including splicing regulation, cell cycle regulation, and DNA damage repair. In our previous study we performed genome-wide identification and functional analysis of SYF2 in plant species. The phylogenetic relationships and expression profiles of SYF2 have not been systematically studied in animals, however. To this end, the gene structure, genes, and protein conserved motifs of 102 SYF2 homologous genes from 91 different animal species were systematically analyzed, along with conserved splicing sites in 45 representative vertebrate species. A differential comparative analysis of expression patterns in humans and mice was made. Molecular bioinformatics analysis of SYF2 showed the gene was conserved and functional in different animal species. In addition, expression pattern analysis found that SYF2 was highly expressed in hematopoietic stem cells, T cells, and lymphoid progenitor cells; in ovary, lung, and spleen; and in other cells and organs. This suggests that changes in SYF2 expression may be associated with disease development in these cells, tissues, or organs. In conclusion, our study analyzes the SYF2 disease resistance genes of different animal species through bioinformatics, reveals the relationship between the SYF2 genotype and the occurrence of certain diseases, and provides a theoretical basis for follow-up study of the relationship between the SYF2 gene and animal diseases.

基因SYF2，作为一种RNA剪接因子，能够在包括剪接调控、细胞周期调控以及DNA损伤修复在内的多种生物过程中与Cyclin D型结合蛋白1（GICP）相互作用。在我方先前的研究中，我们对植物物种中的SYF2进行了全基因组鉴定和功能分析。然而，SYF2在动物中的系统发育关系和表达谱尚未得到系统研究。为此，本研究对来自91种不同动物物种的102个SYF2同源基因的基因结构、基因以及保守的蛋白基序进行了系统分析，同时还包括了45种代表性脊椎动物中的保守剪接位点。通过比较分析人类和老鼠的表达模式，我们进行了差异比较分析。对SYF2的分子生物信息学分析表明，该基因在不同动物物种中均具有保守性和功能性。此外，表达谱分析发现，SYF2在造血干细胞、T细胞和淋巴祖细胞中高表达，在卵巢、肺和脾脏中表达，以及其他细胞和器官中。这表明SYF2表达水平的变化可能与这些细胞、组织或器官的疾病发生有关。总之，本研究通过生物信息学方法分析了不同动物物种的SYF2抗病基因，揭示了SYF2基因型与某些疾病发生之间的关联，并为后续研究SYF2基因与动物疾病之间的关系提供了理论依据。