Transcriptional profiling of Treg cells obtained from lymph nodes of Foxp3GFP-Fth?/? and control Foxp3GFP-Fth?/? mice

To gain further mechanistic insight on how ferritin H chain (FTH) impacts TREG cell lineage maintenance. Overall design: We compared the gene expression profiles of (CD4+GFP+) TREG cells isolated from lymph nodes of Foxp3GFP-Fth?/? (n=5) to control Foxp3GFP-Fth?/? mice (n=5). Mice were bred and maintained under specific pathogen-free (SPF) conditions at the Instituto Gulbenkian de Ciência (IGC). Experimental protocols were approved by the Ethics Committee of the IGC, the “Órgão Responsável pelo Bem-estar dos Animais (ORBEA)” and the Portuguese National Entity (Direcção Geral de Alimentação e Veterinária). Experimental procedures were performed according to the Portuguese (Portaria n° 1005/92, Decreto-Lei n° 113/2013) and European (Directive 2010/63/EU) legislations, concerning housing, husbandry and animal welfare.