20240305 Gray Figure 4 data

FIG 4 Sensitization of E. coli to HOSCN in vivo by copper is slight, and not RclA-dependent. E. coli strains MG1655 (wild-type), MJG1759 (MG1655 ∆copA767::kan+), MJG1958 (MG1655 ∆rclA747), and MJG2358 (MG1655 ∆rclA747 ∆copA767::kan+) were grown in MOPS minimal medium without micronutrients containing 0.2% glucose, supplemented with 1 µM CuCl2 and/or 400 µM HOSCN, as indicated, incubating at 37°C with shaking and measuring A600 at 30-minute intervals for 24 hours (n=4 experimental replicates, with 3 technical replicates per experiment; error bars of 1 standard deviation).