Real-time quantitative PCR analysis of monocytes of major depressed patients [Sertraline study]

Human CD14 positive monocytes were purified from major depressed patients or healthy volunteer frozen PBMC. Patients were treated with Sertralin or Sertralin + Celecoxib. Responders and non-responders to Sertralin and Sertralin + Celecoxib were recorded after 6 weeks of treatment qPCR gene expression profiling. Monocytes prior to treatment were used for qPCR analysis. Details of patient selection, monocyte preparation, recording of patient data can be found in Gara Arteaga Henríquez et al, Frontiers, 2019