Neurodevelopmental Defects and Neurodegenerative Phenotypes in Human Brain Organoids Carrying Parkinson Disease-Linked DNAJC6 Mutations

In this study, human isogenic model of juvenile onset PD caused by DNAJC6 mutation were made. Human ESCs edited using CRISPR-Cas9 to make deleterious DNAJC6 mutation was induced to human midbrain like organoid (hMLO) for studying the mechanism of DNAJC6 deleterious mutation in causing PD Overall design: mRNA profile from 3D organoid Culture of Wildtype and Mutant DNAJC6 midbrain-like Organoid at DIV0, DIV 4, DIV 15, and DIV 30