Effect of restricted glycolysis on gene expression in MDA-MB-231 cells. Effect of restricted glycolysis on gene expression in MDA-MB-231 cells

Cultured breast cancer cells in standard media are highly glycolytic To determine the effect of glycolysis on gene expression in MDA-MB-231 we adapted cells to cuture in mediae containing 10 mM fructose, and compared the gene expression to cells cutured in standard high glucse (25 mM) DMEM Overall design: Cells were adapted to growth in the different sugars for 25 days prior to analysis. Biological replicates represent separately adpated batches of cells.