Effects of ACTH4-10Pro8-Gly9-Pro10 on anti-inflammatory cytokine (IL-4, IL-10, IL-13) expression in acute spinal cord injury model (Sprague Dawley rats)

Background: Spinal cord injury (SCI) is a destructive neurological and pathological state that causes major motor, sensory and autonomic dysfunctions. It’s final neurological outcome determined from both primary and secondary injury process. Neuroinflammation is a key component of the secondary injury mechanisms with local and systemic consequences. A neuroprotective compound, ACTH4-10Pro8-Gly9-Pro10 also known as Semax has shown neuroprotective and anti-inflammatory properties. ACTH4-10Pro8-Gly9-Pro10 also has actively used in the treatment of brain ischemia without serious complication reported. Here we analyzed the effects of ACTH4-10Pro8-Gly9-Pro10 in regulating inflammatory cascade in SCI by looking at the expression of anti-inflammatory cytokine IL-4, IL-10, IL-13 in acute compression SCI.
Method: We do laminectomy in Sprague Dawley rats at the second thoracic vertebrae. After laminectomy we expose the myelum and create mild SCI model with 20gr and severe SCI with 35gr aneurysm cl..., We do laminectomy in Sprague Dawley rats at the second thoracic vertebrae. After laminectomy we expose the myelum and create mild SCI model with 20gr and severe SCI with 35gr aneurysm clips. ACTH4-10Pro8-Gly9-Pro10 was administered intranasally to the treatment group and 0,9% NaCl to the control group (placebo). Both group was remain alive and terminated at 3 and 6 hour. The preparations tissue sample were fixed in formalin and examined for immunohistochemistry. Quantitative measurement of anti-inflammatory cytokine (IL-4, IL-10, IL-13) was done in posterior horn with associated anti-monoclonal antibodies., Microsoft Word; alternatives: Google Docs, Cool Reader, LibreOffice, etc.
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