Genome-wide distribution of H3K9me3, RNA polymerase II, Rloop, DDX55 and HA-tagged DDX55 in WT and DDX55 knockout CD4 naive T cells

Genome-wide distribution of H3K9me3, RNA polymerase II, Rloop, DDX55 and HA-tagged DDX55 in WT and DDX55 knockout CD4 naive T cells were determined by cleavage under targets and tagmentation (CUT&Tag) assays followed by deep sequencing (CUT&Tag-seq) with corresponding antibodies. Cleavage under targets and tagmentation (CUT&Tag) assays were performed using antibodies of H3K9me3, RNA polymerase II, Rloop, DDX55 and HA-tag, respectively on naive T cell isolated from WT and DDX55 knockout mice. The tagmentation products were purified and sequenced on NovaSeq 6000 sequencer (Illumina) with the PE150 model.