Transcriptional profiling of pH-dependent cell cycle progression

Cells have evolved a complex network of signaling cascades that mediate cellular adaptation to availability of nutrients, growth factors or stress. In these networks, cellular information flow is mostly mediated by posttranslational modifications, most notably phosphorylation, or signaling molecules such as GTPases. However, genetic analysis has also implicated a number of proton transporters in regulating cellular signaling processes. Here, we report the transcriptional response of starved cells upon stimulation with serum and glucose upon inhibition of Sodium Hydrogen Exchangers and compare these responses to inhibition of cycle progression with the p300/CBP inhibitor C646. The gene expression profile of starved cells and starved cells stimulated with FCS and glucose for 4 hours in the presence of Dimethyl Amiliride (DMA, 100µM), C646 (10µM) or DMSO only, was compared by Illumina sequencing.