mRNA profiling of AGS Gastric Cancer cell line upon treatment with 5µM of Doxycycline. mRNA profiling of AGS Gastric Cancer cell line upon treatment with 5µM of Doxycycline

Doxycycline, a tetracycline based antibiotic which has bacteriostatic activity against a broad range of both gram positive and gram negative bacteria was identified to inhibit the expression of ERK/MAPK signaling pathway in gastric cancer. ERK/MAPK pathway is one of the highly activated and a crucial player in gastric cancer progression. To delineate the complex transcription program associated with ERK/MAPK pathway and thus inhibited by doxycycline in gastric cancer cells, doxycycline was treated in AGS, one of the ERK/MAPK overexpressed gastric cancer cell line. The resulatant changes in genome-wide mRNA expression pattern between control and doxycycline treated was profiled using Human Transcriptome 2.0 arrays. Overall design: Total RNA was isolated from AGS gastric cancer cell line treated with 5µM of doxycycline and 0.01% of DMSO as a control and profiled using GeneChip® Human Transcriptome Array 2.0 (HTA-2_0).