Single nucleus RNA sequencing reveals heterogeneity among multiple white adipose tissue depots

Regardless of its anatomical site, adipose tissue shares a common energy-storage role but exhibits distinctive properties. Exploring the cellular and molecular heterogeneity of white adipose tissue (WAT) is crucial for comprehending its function and properties. In this study, we employed Single nucleus RNA sequencing (snRNA-seq) to test five representative depots including inguinal, epididymal, mesenteric, perirenal, and pericardial adipose tissues in mice under physiological conditions. By analyzing the contents of main cell categories and gene profiles of various depots, we identified their distinctive physiological properties. Overall design: To construct the atlas of cell types in different adipose tissue depots, we performed snRNAseq of epididymal white adipose tissue (EWAT), subcutaneous white adipose tissue (SWAT), mesenteric white adipose tissue (MWAT), perirenal white adipose tissue (NWAT) and peri-heart white adipose tissue (HWAT) pooled from mice fed chow diet for analysis.