Microarray transcriptome analysis of primary mouse microglia cells treated with NGF

The microarray transcriptome analysis of primary microglia was performed to demonstrate a direct modulation of NGF activity on microglia cells. The three different time points were used to evaluate and describe the time course of genes patwhays. In summary this experiment was crucial to describe for the first time the kind of NGF activity on microglia. A total of n=14 samples were processed: 2 experimental groups (NGF-treated and untreated control) at 3 different time points (2h, 8h, 24h). Two biological replicates were processed for each experimental point (treatment,time) except controls at 24h (4 replicates).