RIP-seq reveals the interactome of nucleolin in activated CD4+ T cells

During the actiation of CD4+T cells, the pri-miRNAs translocated from cytoplasma into nucleus.However, the mechanism remains unkown. We found that pri-miR-31 interact with nucleolin through G-guadruplex struction in pri-miR-31. We then transfected ASO to destribute the interaction of nucleolin and G4 struction. In order to identify the interacting RNA, including miRNA precuser and mRNA, we performed the RIP-seq Immunoprecipited by anti-nucleolin and IgG. Overall design: Naïve CD4+ T cells derived from wild type C57 mice were activated by anti-CD3/28. Cells were lysated and Immunoprecipitated by antinucleolin and IgG. Paired reads were mapping to mm10 gemonic.